Orphaned as a small child. Raised in the Torlonia Conservatory on Via Sant’ Onofrio, under the guidance of the Daughters of Charity of Saint Vincent de Paul. Entered the Daughters’ novitiate in Siena, Italy, but was forced to leave due to poor health.
On retreat in 1891 she met Blessed Louis Tezza, procurator general of the Camillians. He had been thinking of founding a women’s community for the care of the sick. He invited Josephine to help establish the new community, she prayed over it, and decided “yes.” In 1892 she and two companions received the scapular of Camillian tertiaries, and a year later professed private vows, adding service to the sick, even at risk of their lives. They took their perpetual vows in 1895, and Josephine was elected Superior General. Blessed Louis was sent to Lima, Peru in 1900, responsibility for the new congregation rested with Mother Vannini, and under her leadership the congregation spread to France, Belgium and Argentina.